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Specificity of autoantibodies for recombinant 60-kd and 52-kd Ro autoantigens
E W St Clair1, J A Burch, M Saitta
1Duke University Medical Center, Durham, NC 27710.
Objective:
To compare patterns of Ro autoantigen recognition in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE).
Methods:
Sera were tested by enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation for reactivity with recombinant human 60-kd and 52-kd Ro proteins.
Results:
SLE and primary SS sera exhibited similar patterns of reactivity with the recombinant 52-kd protein. However, a greater proportion of sera from primary SS patients than from SLE patients did not react with recombinant 60-kd protein in ELISA yet precipitated its translation product in solution-phase assays.
Conclusion:
Disease state can influence recognition of the recombinant 60-kd Ro antigen.