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Transgenic animal models for measuring mutations in vivo
J C Mirsalis1, J A Monforte, R A Winegar
1SRI International, Toxicology Laboratory, Menlo Park, CA 94025-3493.
Critical Reviews in Toxicology
|January 1, 1994
Summary
Transgenic animal models using reporter genes like lacI or lacZ enable rapid measurement of tissue-specific mutations after in vivo treatments. These models are crucial for assessing genotoxicity and guiding future research.
Area of Science:
- Genetics and Genomics
- Toxicology and Pharmacology
- Biotechnology
Background:
- Transgenic animal models are essential for in vivo mutation detection.
- Reporter genes such as Escherichia coli lacI and lacZ are commonly used.
- These models facilitate rapid assessment of tissue-specific mutations.
Purpose of the Study:
- To review methods for creating and utilizing transgenic models for mutation measurement.
- To discuss gene rescue, sequencing, and study design parameters.
- To summarize existing data and propose future research directions.
Main Methods:
- Utilizing transgenic rodents with integrated lacI or lacZ reporter genes.
- Isolating genomic DNA from various tissues post-in vivo treatment.
- Packaging target genes into lambda-phage for E. coli infection and plaque analysis.
- Detecting mutations via colorimetric or selective assays.
Main Results:
- Established methodologies for producing and applying transgenic mutation detection systems.
- Detailed techniques for gene rescue, mutant sequencing, and experimental design.
- Compilation of published data on the efficacy of these transgenic models.
Conclusions:
- Transgenic models offer a powerful platform for in vivo genotoxicity assessment.
- Standardized methods enhance the reliability of mutation detection across tissues.
- Further research is needed to optimize dosing and study designs for comprehensive genotoxicity evaluation.