Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Is the circulating ouabain-like compound ouabain?

E P Gomez-Sanchez1, M F Foecking, D Sellers

  • 1Research Service, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65201.

American Journal of Hypertension
|July 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Improving cosmological reach of a gravitational wave observatory using Deep Loop Shaping.

Science (New York, N.Y.)·2025
Same author

Squeezing the quantum noise of a gravitational-wave detector below the standard quantum limit.

Science (New York, N.Y.)·2024
Same author

Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

Physical review letters·2022
Same author

Point Absorber Limits to Future Gravitational-Wave Detectors.

Physical review letters·2021
Same journal

Efficacy and Safety of Baxdrostat in the Management of Uncontrolled Hypertension: A Systematic Review and Meta-Analysis.

American journal of hypertension·2026
Same journal

Hemodynamic Evolution of Heart Failure from Hypertension: Lower Stroke Volume Index and Higher Heart Rate.

American journal of hypertension·2026
Same journal

Opposite Seasonal Variation in Nighttime and Morning Blood Pressure: A Longitudinal Study of Patients with Hypertension.

American journal of hypertension·2026
Same journal

Screening for Postprandial Hypotension: The Importance of Home Blood Pressure Monitoring.

American journal of hypertension·2026
Same journal

Distinct Mediating Roles of Hypertension in Arterial Stiffness Mechanisms Associated with Cardiovascular Disease Risk: A Community-Based Cohort Study.

American journal of hypertension·2026
Same journal

Long-term systolic blood pressure time in target and risk of cardiovascular events in older adults: a secondary analysis of the ASPREE cohort.

American journal of hypertension·2026
See all related articles

Researchers investigated circulating sodium, potassium ATPase inhibitors in hypertension. Their sensitive immunoassay detected minimal ouabain, suggesting the inhibitor is not ouabain but a similar compound.

Area of Science:

  • Cardiovascular Physiology
  • Endocrinology
  • Biochemistry

Background:

  • Volume-expanded hypertension is linked to a circulating inhibitor of sodium, potassium ATPase.
  • Ouabain was previously identified as this inhibitor in human plasma.

Purpose of the Study:

  • To investigate the presence and identity of the circulating sodium, potassium ATPase inhibitor in plasma.
  • To validate or refute the identification of ouabain as the endogenous inhibitor.

Main Methods:

  • Development and application of a highly specific and sensitive immunoassay for ouabain.
  • Immunoassay of human and rat plasma, and adrenal cell culture extracts.
  • High-Performance Liquid Chromatography (HPLC) separation and analysis.
  • Assay of sodium, potassium ATPase activity.

Related Experiment Videos

Main Results:

  • The immunoassay detected very low or undetectable levels of immunoreactive ouabain in plasma and adrenal extracts.
  • Authentic ouabain was detectable at low concentrations when added to the extracts.
  • Sodium, potassium ATPase activity was present in adrenal cell culture extracts.
  • A small amount of immunoassayable ouabain was detected in bovine adrenal extracts, but it did not correspond to the authentic ouabain elution time.

Conclusions:

  • The circulating sodium, potassium ATPase inhibitor in volume-expanded hypertension is unlikely to be ouabain.
  • The endogenous inhibitor is likely structurally similar to ouabain, indicated by cross-reactivity with ouabain antibodies.