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Related Experiment Videos

How to diagnose polysulphide poisoning from tissue samples

T Nagata1, S Kage, K Kimura

  • 1Department of Forensic Medicine, Faculty of Medicine, Kyushu University 60, Fukuoka, Japan.

International Journal of Legal Medicine
|January 1, 1994
PubMed
Summary

This study confirms polysulphide poisoning in rats by measuring polysulphides and their toxic byproduct, sulphide, in tissues. Blood analysis is key for diagnosing polysulphide poisoning.

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Area of Science:

  • Toxicology
  • Analytical Chemistry

Background:

  • Polysulphides are used in bathing agents.
  • Toxicological data on polysulphide poisoning is limited.

Observation:

  • Animal experiments were conducted using rats orally administered a calcium polysulphide bathing agent.
  • Tissue samples were analyzed for polysulphides and sulphide using Gas Chromatography (GC) and GC/Mass Spectrometry (GC/MS).

Findings:

  • Polysulphides were highest in blood (0.196 mumol/ml), followed by liver, lungs, and kidneys.
  • Sulphide, a decomposition product, was detected in all tissues, with blood showing the highest concentration (0.196 mumol/ml), significantly exceeding lethal levels for hydrogen sulphide.
  • Polysulphide poisoning can be diagnosed by detecting polysulphides and sulphide in body tissues, particularly blood.

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Implications:

  • Establishes a method for diagnosing polysulphide poisoning.
  • Highlights the critical role of blood analysis in toxicological assessments of polysulphide exposure.
  • Provides crucial data for understanding the toxicokinetics and diagnosis of polysulphide poisoning in clinical settings.