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Ferrous sulphate does not directly affect pteroylmonoglutamic acid absorption in rats
N R Campbell1, B B Hasinoff, M Singh
1Department of Medicine, Faculty of Medicine, University of Calgary, Alberta, Canada.
The British Journal of Nutrition
|September 1, 1994
Summary
Iron (Fe) supplements do not significantly affect the absorption or stability of pteroylmonoglutamic acid. In vitro and in vivo studies found no evidence of complex formation impacting nutrient absorption.
Area of Science:
- Nutritional Biochemistry
- Pharmacokinetics
- Inorganic Chemistry
Background:
- Iron (Fe) compounds can reduce the absorption of co-administered substances.
- Pteroylmonoglutamic acid is a form of folate crucial for various biological processes.
Purpose of the Study:
- To investigate the in vitro binding of Fe2+ and Fe3+ ions to pteroylmonoglutamic acid.
- To assess the in vivo effect of FeSO4 on pteroylmonoglutamic acid absorption in rats.
Main Methods:
- In vitro complex formation studies using dimethylsulphoxide (DMSO) and DMSO-aqueous Bis-Tris buffer.
- In vivo studies utilizing an isolated perfused rat jejunal model to measure nutrient absorption.
Main Results:
- In vitro, pteroylmonoglutamate formed a 2:1 complex with Fe3+ in DMSO alone, but not in aqueous buffer.
- Spectroscopic evidence confirmed Fe2+ complex formation in DMSO alone, but not in the aqueous buffer.
- In vivo, FeSO4 did not significantly affect pteroylmonoglutamic acid absorption or stability in the rat jejunal model.
Conclusions:
- Complex formation between pteroylmonoglutamic acid and iron ions is dependent on the solvent system.
- Concurrent ingestion of iron preparations is unlikely to significantly reduce pteroylmonoglutamic acid absorption.
- These findings suggest minimal pharmacokinetic interaction between iron and pteroylmonoglutamic acid in vivo.