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Quantitative analysis of superoxide anion generation in living cells by using chemiluminescence video microscopy
1Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Biochimica Et Biophysica Acta
|November 11, 1994
Abstract:
Superoxide anions (O2-) generated by rabbit neutrophils were detected and quantified by a video microscope equipped with a photon-counting camera. One count obtained by this system was equivalent to 59 amol of O2-. Maximum O2- production was observed at 6-8 min after stimulation and was estimated as 1.9 fmol/min per cell on the average.