Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucocorticoid hormone binding to rat adipocytes

J Sjögren1, M Weck, A Nilsson

  • 1Wallenberg Laboratory, Department of Heart and Lung Diseases, Sahlgren's Hospital, University of Göteborg, Sweden.

Biochimica Et Biophysica Acta
|October 20, 1994
PubMed
Summary

This study quantified glucocorticoid receptor (GR) binding in intact rat adipocytes, revealing higher GR density in intra-abdominal fat depots, particularly mesenteric tissue. This finding may explain the heightened activity of glucocorticoid-regulated metabolic pathways in these regions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How carbon monoxide adsorbs in different sites.

Physical review letters·2000
Same author

Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract.

Chemistry and physics of lipids·2000
Same author

Wheezing following lower respiratory tract infections with respiratory syncytial virus and influenza A in infancy.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2000
Same author

Allopurinol in addition to 5-aminosalicylic acid based drugs for the maintenance treatment of ulcerative colitis.

Alimentary pharmacology & therapeutics·2000
Same author

Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure.

Journal of chromatography. B, Biomedical sciences and applications·2000
Same author

Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries.

Nature biotechnology·2000

Area of Science:

  • Endocrinology
  • Cell Biology
  • Metabolic Research

Background:

  • Previous studies quantified glucocorticoid receptor (GR) binding in adipose tissue cytosol, limiting the determination of total cellular GR.
  • Intact adipocytes offer a more accurate model for assessing total GR binding capacity.

Purpose of the Study:

  • To quantify GR binding in intact adipocytes from different rat adipose tissue depots.
  • To compare GR binding capacity across epididymal, retroperitoneal, inguinal, and mesenteric fat tissues.
  • To investigate the influence of cellular density on GR distribution.

Main Methods:

  • Isolated adipocytes from male rat epididymal, retroperitoneal, inguinal, and mesenteric adipose tissues.
  • Used radiolabeled dexamethasone (dex) as a ligand to measure GR binding.

Related Experiment Videos

  • Performed experiments on intact adipocytes and after adrenalectomy (ADX) or culture in a glucocorticoid-free medium.

    • Main Results:

    • GR binding was saturable and specific, with a dissociation constant (Kd) in the nanomolar range.
    • Binding capacity increased after removal of endogenous glucocorticoids (ADX or culture).
    • Mesenteric adipose cells exhibited higher GR binding capacity per triglyceride weight, attributed to greater cellular density.
    • Intra-abdominal adipocytes (epididymal, retroperitoneal, mesenteric) showed higher GR binding per cell compared to subcutaneous (inguinal) adipocytes.

    Conclusions:

    • Mesenteric adipose tissue possesses a higher in situ GR density, primarily due to increased cellular density.
    • Intra-abdominal adipocytes generally exhibit higher GR density than subcutaneous adipocytes.
    • Elevated GR density in intra-abdominal fat may contribute to the high activity of glucocorticoid-regulated metabolic pathways in these depots.