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Updated: Jul 20, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Quantification of GR90291 in human blood by high resolution gas chromatography-mass selective detection (HRGC-MSD)
D Lessard1, B Comeau, A Charlebois
1Phoenix International Life Sciences, Saint-Laurent, Montreal, Canada.
Abstract:
A specific and sensitive high resolution gas chromatography-mass spectrometry method for the determination of GR90291 in human blood is described. The extraction of GR90291 from blood required a polar organic solvent mixture. The crude extract was further purified by successive liquid-liquid partitioning prior to esterification with an HCl-n-butanol solution. This derivative was analysed using a deuterium-labelled internal standard by selected ion monitoring mass spectrometry. The calibration curve ranged from 1 to 100 ng ml-1. The method is reliable for the determination of GR90291 pharmacokinetics in human subjects.
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