Biochemical and pathological observations on sheep showing various clinical manifestations of experimental...

For observing chronological changes of biochemical and pathological aspects in experimental cerebrocortical necrosis, 14 sheep were given amprolium (600 mg/kg/day), and killed at various clinical stages. At the onset of abnormal electroencephalograms, total thiamine concentrations in the cerebrum, liver, heart and blood were reduced significantly. After anorexia was noted, total thiamine concentrations in them did not decrease further as symptoms developed severely. Autofluorescence and necrotic lesions in the cerebral cortex were observed in animals killed one day after the onset of abnormal electroencephalograms.