[Direct detection of an antigen in immunoglobulin G Langmuir-Blodgett films based on a surface plasma resonance method and in a piezoelectric system]

The possibilities of direct antigen detection in unlabelled systems based on immunoglobulin G Langmuir-Blodgett films as a sensitive surface have been studied. It was shown that an increase in the monolayer number in an immunoglobulin G Langmuir-Blodgett film deposited onto a solid surface coated with a thin silver film (50 nm) resulted in the regeneration of the antigen-binding capacity of the upper antibody layer. This dependence can be used for the construction of a direct optical immunosensor based on surface plasmon resonance. Moreover, a model of a piesoelectric immunosensor on the basis of immunoglobulin G Langmuir-Blodgett films for direct ferritin detection has been proposed. The detection range of ferritin concentrations in solution is 10(-10)-10(-7) M.