Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Forms and functions of p53

J Milner1

  • 1Department of Biology, University of York, UK.

Seminars in Cancer Biology
|June 1, 1994
PubMed
Summary
This summary is machine-generated.

Wild-type p53 protein exists in multiple forms that control cell growth. Understanding these p53 conformations offers new anti-cancer therapy strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing methods for aggregating indoor air pollutant concentration over space and time.

Journal of physics. Conference series·2026
Same author

Five-year study assessing the clinical utility of anti-Müllerian hormone measurements in reproductive-age women with cancer.

Reproductive biomedicine online·2019
Same author

Household air pollution in Nairobi's slums: A long-term policy evaluation using participatory system dynamics.

The Science of the total environment·2019
Same author

Density of GFAP-immunoreactive astrocytes is decreased in left hippocampi in major depressive disorder.

Neuroscience·2016
Same author

Identification of LDH-A as a therapeutic target for cancer cell killing via (i) p53/NAD(H)-dependent and (ii) p53-independent pathways.

Oncogenesis·2014
Same author

Nutrition economics - food as an ally of public health.

The British journal of nutrition·2013
Same journal

Role of oral microbiome in cancer immunotherapy.

Seminars in cancer biology·2026
Same journal

Unveiling tumor heterogeneity by single cell RNA-sequencing: From basic considerations to clinical applications.

Seminars in cancer biology·2026
Same journal

Hidden architecture of resistance: The extracellular matrix in melanoma's immune landscape.

Seminars in cancer biology·2026
Same journal

Microfluidic technologies for extracellular vesicle isolation and analysis: Implications for translational applications in cancer therapy.

Seminars in cancer biology·2026
Same journal

Exploring glioblastoma microenvironment using organoids: opportunities, limitations, and emerging concepts.

Seminars in cancer biology·2026
Same journal

Special issue: Lipid metabolic alterations in cancer: Pathogenic mechanisms, therapies and common pathophysiology with cardiovascular disease.

Seminars in cancer biology·2026
See all related articles

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Wild-type p53 protein exhibits dual functions in cell proliferation, promoting and suppressing growth.
  • These opposing functions are linked to distinct conformations of the p53 protein.

Purpose of the Study:

  • To propose that wild-type p53 adopts at least three distinct forms.
  • To correlate these forms with specific functions in cell growth control.
  • To identify novel anti-cancer therapy rationales based on p53 structure regulation.

Main Methods:

  • Characterization of p53 protein forms using monoclonal antibodies PAb421 and PAb1620.
  • Analysis of p53 tertiary structure modulation through redox and phosphorylation.

Main Results:

Related Experiment Videos

  • Identified three distinct p53 forms defined by reactivity with PAb421 and PAb1620.
  • Form 1 (421-/1620+) suppresses cell growth and maintains quiescence.
  • Form 2 (421+/1620-) activates and promotes cell proliferation.
  • Form 3 (421+/1620+) acts as a sensor for growth regulatory signals, convertible to suppressor or promoter forms.

Conclusions:

  • Wild-type p53's diverse functions are mediated by at least three distinct conformational states.
  • Regulation of p53 tertiary structure via redox modulation and phosphorylation provides a basis for anti-cancer drug development.