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Optimization by mass spectrometry of a tryptophan-specific protein cleavage reaction
M M Vestling1, M A Kelly, C Fenselau
1Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore.
Rapid Communications in Mass Spectrometry : RCM
|September 1, 1994
Abstract:
Mass spectrometry has been used to define the reaction of BNPS-skatole (3-bromo-3-methyl-2-(o-nitrophenylsulfenyl)indolenine) with selected proteins and peptides, confirming the cleavage of the protein chain at tryptophan residues, characterizing the products, and identifying the side reactions. A short procedure for carrying out the cleavage reaction with minimal side reactions is presented.