Evaluation of three oil-adjuvant vaccines against Pasteurella multocida in buffalo calves

Three oil-adjuvant vaccines of Pasteurella multocida 6:B were evaluated with respect to the level and duration of the humoral immune response produced in buffalo calves. Preparation 1 was a water-in-oil emulsion containing Marcol 52, Montanide 888 and antigen at a ratio of 6:1:3. Preparation 2 was a double emulsion containing Marcol 52, Arlacel A and Tween 80 in addition to antigen. Preparation 3 contained alpha-d-tocopheryl acetate (vitamin E), Montanide 888 and antigen. All three preparations induced a similar sustained immune response in buffalo calves beyond 270 days post-vaccination.