Related Experiment Video

Updated: Aug 9, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Transactivation of the human multidrug resistance (MDR1) gene promoter by p53 mutants

K T Nguyen1, B Liu, K Ueda

  • 1Cancer Institute of New Jersey, Piscataway.

Oncology Research
|January 1, 1994
PubMed

Insights

The p53 tumor suppressor influences multidrug resistance (MDR) by affecting the MDR1 gene promoter. This suggests a link between cancer-driving gene mutations and drug resistance mechanisms.

Area of Science:

  • Molecular Biology
  • Cancer Genetics

Background:

  • Multidrug resistance (MDR) in human cancers is often linked to the MDR1 gene, which encodes P-glycoprotein, an efflux pump conferring resistance to chemotherapy drugs.
  • Previous studies indicated that the MDR1 gene promoter's activity is modulated by Ras and p53, suggesting a role in oncogenesis.

Purpose of the Study:

  • To further investigate the specific effects of the p53 tumor suppressor protein on the human MDR1 gene promoter.
  • To characterize the interaction between p53 mutants and the MDR1 promoter region essential for its activity.

Main Methods:

  • Utilized cotransfection experiments to study gene promoter activity.
  • Employed gene targeting to generate p53-deficient mice.
  • Analyzed p53 mutant transactivation of the MDR1 promoter in various cell types, including mouse embryo fibroblasts.

Main Results:

  • The promoter region critical for basal MDR1 activity is also required for transactivation by p53 mutants.
  • Multiple distinct p53 mutants were shown to activate the MDR1 promoter.
  • This activation was observed across several different cell types.

Conclusions:

  • p53, a key tumor suppressor, directly impacts the MDR1 gene promoter, influencing multidrug resistance.
  • The findings suggest a mechanistic link between tumor suppressor gene function/dysfunction and the development of chemotherapy resistance in cancer.

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...