Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Programmed cell death in Caenorhabditis elegans

M O Hengartner1, H R Horvitz

  • 1Cold Spring Harbor Laboratory, New York.

Current Opinion in Genetics & Development
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Essential versus accessory aspects of cell death: recommendations of the NCCD 2015.

Cell death and differentiation·2014
Same author

Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans.

Cell death and differentiation·2014
Same author

Differential regulation of DNA damage response activation between somatic and germline cells in Caenorhabditis elegans.

Cell death and differentiation·2012
Same author

PaxDb, a database of protein abundance averages across all three domains of life.

Molecular & cellular proteomics : MCP·2012
Same author

Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012.

Cell death and differentiation·2011
Same author

A lectin-mediated resistance of higher fungi against predators and parasites.

Molecular ecology·2011

Programmed cell death in nematodes depends on ced-3 and ced-4 genes, and is opposed by ced-9. This research reveals a shared cell death pathway between nematodes and mammals.

Area of Science:

  • * Molecular biology
  • * Developmental biology
  • * Genetics

Background:

  • * Programmed cell death, or apoptosis, is a fundamental biological process crucial for development and tissue homeostasis.
  • * The nematode Caenorhabditis elegans is a powerful model organism for studying conserved biological pathways.
  • * Key genes regulating apoptosis in C. elegans, including ced-3, ced-4, and ced-9, have been identified.

Purpose of the Study:

  • * To investigate the genetic requirements for programmed cell death in Caenorhabditis elegans.
  • * To clone and characterize the genes ced-3, ced-4, and ced-9 involved in apoptosis.
  • * To determine the evolutionary conservation of the cell death pathway between nematodes and mammals.

Main Methods:

  • * Genetic analysis of programmed cell death mutants in C. elegans.

Related Experiment Videos

  • * Molecular cloning of the ced-3, ced-4, and ced-9 genes.
  • * Sequence analysis of the cloned genes and comparison with known vertebrate cell death genes.

    • Main Results:

    • * Programmed cell death in C. elegans requires the ced-3 and ced-4 genes and is antagonized by ced-9.
    • * Cloning of these genes revealed that ced-3 and ced-4 encode proteins with similarity to vertebrate apoptosis-regulating proteins.
    • * The findings indicate a shared molecular machinery for programmed cell death across species.

    Conclusions:

    • * The core components of the programmed cell death pathway are conserved between nematodes and mammals.
    • * This conservation highlights the fundamental nature of apoptosis and its genetic regulation.
    • * C. elegans serves as a valuable model for understanding human cell death mechanisms.