Purification of a maize dehydrin
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Updated: Jan 26, 2026

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
Published on: September 15, 2023
T L Ceccardi1, N C Meyer, T J Close
1Department of Botany and Plant Sciences, University of California, Riverside 92521-0124.
Abstract:
A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89 degrees C and centrifuged to remove heat-insoluble proteins. The remaining soluble proteins were fractionated in a three-step chromatographic process. Following cation exchange, hydrophobic interaction, and gel filtration chromatography, pure dehydrin samples were obtained.
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