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Updated: Aug 5, 2026

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Published on: July 3, 2013
Potential-dependent Ca2+ currents in isolated heart cells of hibernators
A E Alekseev1, A F Korystova, D A Mavlyutova
1Laboratory of Functional Biochemistry, Russian Academy of Sciences, Pushchino, Moscow Region.
Abstract:
Using the perforated patch-clamp technique, it has been shown that the potential-dependent Ca2+ current in cardiac myocytes of hibernators is not completely inhibited during hibernation. The residual Ca2+ current is classed as L-type Ca2+ current which is regulated by agonists and antagonists of Ca2+ channels. The effect of nifedipine, D600, isoproterenol and forskolin has been examined. It was found that isoproterenol affects the affinity of Ca2+ channels for the D600 blocker.
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