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Cross-linking of dermal collagen induced by singlet oxygen
T Hikichi1, N Ueno, C L Trempe
1Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114.
Biochemistry and Molecular Biology International
|June 1, 1994
Abstract:
Changes were studied in dermal collagen induced by singlet oxygen, which was generated by a bilirubin-photosensitized reaction. An increase in the ratio of high-molecular-weight components (beta- and r-components) against the alpha-chain of collagen after irradiation manifested in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sodium azide, a singlet oxygen quencher, inhibits the alteration of the molecular size by the photochemical reaction. These data suggest that chronic sunlight irradiation may contribute to cross-links of dermal collagen.