[Effect of histamine on behavior of the rosette test in patients with pollinosis]

The study comprised of 50 patients with the diagnosis of pollinosis based on characteristic history data, positive results of skin test and positive IgE FAST test with grass pollen allergens. 20 ml of blood was taken from all patients and total E rosette test was carried. Antihistamine was added to rosette test in the concentration of 10(-3) mg/ml in the following doses: 0.1 ml, 0.2 ml, 0.3 ml. Histamine caused stat. sig. (P < 0.001) decrease in the percentage of lymphocytes forming E rosettes. We think that lymphocyte may be injured by histamine under exposition to grass pollens.