Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation and characterization of hypervariable sequences from tsetse fly genome

A Blanchetot1, R H Gooding

  • 1Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.

Insect Biochemistry and Molecular Biology
|October 1, 1994
PubMed
Summary

Researchers developed DNA probes to study genetic variation in tsetse flies. These probes identified both single and multiple DNA locations, revealing low genetic variability in an inbred colony.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The tsetse fly Glossina palpalis palpalis is composed of several genetically differentiated small populations in the sleeping sickness focus of Bonon, Côte d'Ivoire.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2006
Same author

Identification of midgut proteins that are differentially expressed in trypanosome-susceptible and normal tsetse flies (Glossina morsitans morsitans).

Insect biochemistry and molecular biology·2005
Same author

Tsetse genetics: contributions to biology, systematics, and control of tsetse flies.

Annual review of entomology·2004
Same author

Monitoring the susceptibility of Glossina palpalis gambiensis and G. morsitans morsitans to experimental infection with savannah-type Trypanosoma congolense, using the polymerase chain reaction.

Annals of tropical medicine and parasitology·2004
Same author

PCV2 replicase transcripts in infected porcine kidney (PK15) cells.

Virus genes·2003
Same author

The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia.

Insect biochemistry and molecular biology·2003

Area of Science:

  • Genomics
  • Molecular Biology
  • Entomology

Background:

  • The tsetse fly genome presents unique challenges for genetic analysis.
  • Developing polymorphic DNA markers is crucial for understanding tsetse fly population genetics and evolution.

Purpose of the Study:

  • To construct and characterize a genomic library of Glossina brevipalpis.
  • To identify and validate DNA probes that reveal polymorphisms in the tsetse fly genome.
  • To assess genetic variability within tsetse fly populations using these probes.

Main Methods:

  • Construction of a Glossina brevipalpis genomic library using a Charomid 9-36 vector.
  • Screening of 300 clones with five types of polymorphic DNA probes.
  • Characterization of 24 recombinant clones using Southern blotting hybridization with DNA from Glossina morsitans centralis.

Related Experiment Videos

  • Analysis of DNA profiles (multilocus and single locus) and genetic variability.

    • Main Results:

    • 15% of screened clones hybridized with at least one probe.
    • Two classes of DNA profiles were identified: multilocus and single locus.
    • 13 multilocus and 11 single locus probes were characterized.
    • Single locus probes revealed low genetic variability in an inbred G. m. centralis colony.
    • Some probes detected polymorphisms in Glossina palpalis palpalis, Glossina palpalis gambienis, honey bees, and humans.

    Conclusions:

    • The study successfully generated and validated polymorphic DNA probes for tsetse flies.
    • These probes are valuable tools for studying genetic diversity and population structure in tsetse flies and potentially other species.
    • The findings indicate low genetic variability in the tested inbred tsetse fly colony.