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[A new antigen induced by oncornavirus D in mouse fibroblast culture]
Abstract:
It was established by the CFT and immunofluorescence that oncornavirus D inoculated into continuous mouse fibroblast cultures induced in them a new antigen differing from those of the virus and of the original non-infected cells. The antigen was shown to accumulate during cultivation of the infected cells. No antigen was induced by oncornavirus inactivated by heating or neutralized by antiviral antiserum. The physico-chemical and immunological properties of the new antigen experimentally induced in the mouse fibroblast culture are identical to those of the new antigen present in cultures of human J-96, HeLa and HEp-2 cells.
Insights
Oncornavirus D infection of mouse cells creates a new antigen, distinct from viral or cell components. This induced antigen
Area of Science:
- Virology and Immunology
- Cell Biology
- Oncornavirus Research
Background:
- Oncornaviruses are known to interact with host cells.
- Cellular responses to viral infections can involve the expression of new antigens.
- Previous studies have identified new antigens in human cell lines infected with certain viruses.
Purpose of the Study:
- To investigate the induction of new antigens in mouse fibroblast cultures following oncornavirus D inoculation.
- To characterize the properties of the induced antigen.
- To compare the induced antigen with antigens found in human cell lines.
Main Methods:
- Complement fixation test (CFT) and immunofluorescence were used to detect and characterize the antigen.
- Mouse fibroblast cultures were inoculated with oncornavirus D.
- Oncornavirus was inactivated by heating or neutralized by antiserum to assess antigen induction specificity.
- Physico-chemical and immunological properties of the induced antigen were analyzed.
Main Results:
- Oncornavirus D inoculation induced a new antigen in mouse fibroblast cultures.
- The induced antigen differed from the virus and original host cell antigens.
- Antigen accumulation was observed during the cultivation of infected cells.
- Inactivated or neutralized oncornavirus did not induce the antigen.
- The induced antigen exhibited identical physico-chemical and immunological properties to those found in human cell lines (J-96, HeLa, HEp-2).
Conclusions:
- Oncornavirus D actively induces a specific new antigen in mouse fibroblast cells.
- The induction of this antigen is dependent on the infectivity of the oncornavirus.
- The newly induced antigen in mouse cells shares characteristics with similar antigens in human cell lines, suggesting conserved mechanisms across species.