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High-performance capillary electrophoretic analysis of inflammatory cytokines in human biopsies
1Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Abstract:
The detection of inflammatory cytokines in pathological tissue samples is important as an indicator of disease severity and persistence. Capillary zone electrophoresis (CZE) has been shown to be a good technique for the detection and measurement of tissue-bound cytokines in frozen biopsy specimens. CZE separations in polyethylene glycol-coated capillaries, performed at neutral pH and sub-ambient temperatures was found to give the most consistent and reliable results.