Separation of fifteen non-steroidal anti-inflammatory drugs using micellar electrokinetic capillary chromatography

Micellar electrokinetic capillary chromatography with sodium dodecylsulfate as anionic surfactant was used to separate simultaneously fifteen non-steroidal anti-inflammatory drugs. UV detection was performed at 254 nm. The electroosmotic flow was carefully adjusted for optimal separation. Resolution of the drugs was obtained using a buffer containing 40 mmol l-1 NaH2PO4, 0.104 mol l-1 sodium dodecylsulfate and 3% (v/v) methanol, adjusted to pH 8 with sodium hydroxide. Lack of interference was checked with a number of drugs and metabolites. Between-day coefficients of variation ranged between 2 to 10%.