Related Experiment Video
Updated: Aug 12, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Determination of ascorbic acid and uric acid in plasma by high-performance liquid chromatography
1Rowett Research Institute, Bucksburn, Aberdeen, UK.
Abstract:
A simple method with excellent reproducibility has been developed for the simultaneous determination of the plasma concentration of ascorbic acid and uric acid by reversed-phase HPLC using an ion-pairing reagent with UV detection. Coefficients of variation for the concentrations of ascorbic acid and uric acid in plasma are < 2.1% and < 3.5% respectively.
More Related Videos
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020
05:03Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
Related Concept Videos
Polyprotic Acids
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process: