Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunofluorescence on resin-embedded material

J Scotto

    Histopathology
    |September 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Preserving antigenic determinants in liver tissue after plastic embedding is possible. This method allows for correlative light and electron microscopy of immunofluorescence results.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mood symptoms and suicidality across the autism spectrum.

    Comprehensive psychiatry·2019
    Same author

    Correlations between standard auditory evoked potentials and symptomatology in a group of 50 schizophrenic patients.

    European psychiatry : the journal of the Association of European Psychiatrists·2009
    Same author

    Melanoma: linked temporal and latitude changes in the United States.

    Cancer causes & control : CCC·1993
    Same author

    Indications of future decreasing trends in skin-melanoma mortality among whites in the United States.

    International journal of cancer·1991
    Same author

    Incidence of nonmelanoma skin cancer in New Hampshire and Vermont.

    Journal of the American Academy of Dermatology·1991
    Same author

    Effects of polyunsaturated fatty acids on human and rat cells. In vitro versus in vivo experiments.

    In vivo (Athens, Greece)·1989
    Same journal

    TROP2 immunoreactivity in pulmonary large cell neuroendocrine carcinoma.

    Histopathology·2026
    Same journal

    Malignant adenomyoepithelioma of the breast: seven cases illustrating morphological diversity and diagnostic challenges.

    Histopathology·2026
    Same journal

    A CRX-positive RB1-deficient bone tumour with a retinoblastoma-like DNA methylation profile.

    Histopathology·2026
    Same journal

    Perivascular epithelioid cell tumours of the genitourinary tract: clinicopathological features and molecular landscape.

    Histopathology·2026
    Same journal

    Navigating diagnostic challenges in low-grade spindle cell lesions of the breast: a retrospective review.

    Histopathology·2026
    Same journal

    Neoplastic transformation of sporadic gastric hyperplastic polyps: a systematic review and meta-analysis of risk factors and clinicopathological features.

    Histopathology·2026
    See all related articles

    Area of Science:

    • Immunohistochemistry
    • Electron Microscopy
    • Molecular Biology

    Background:

    • Antigenic determinants can be preserved in plastic-embedded tissues.
    • Correlative microscopy requires compatible tissue preparation methods.

    Purpose of the Study:

    • To evaluate a method for preserving antigenic determinants in embedded liver tissue.
    • To enable correlative light and electron microscopy of immunofluorescence.

    Main Methods:

    • Liver tissue fixation with glutaraldehyde or paraformaldehyde.
    • Dehydration and embedding in araldite with polymerization at 37°C.
    • Immunofluorescence on hydrogen peroxide-etched semi-thin sections.

    Main Results:

    • Successful preservation of antigenic determinants was achieved.

    Related Experiment Videos

  • The method allowed for correlation between light and electron microscopy.
  • Effective immunofluorescence was observed using various specific antisera.

    • Conclusions:

    • Plastic embedding preserves antigenic determinants in liver tissue.
    • This technique facilitates correlative microscopy for detailed analysis.
    • The method is suitable for studying various liver antigens.