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Computer program for the analysis of mutational spectra: application to p53 mutations

N F Cariello1, W W Piegorsch, W T Adams

  • 1University of North Carolina, Pathology Department, Chapel Hill 27599-7525.

Carcinogenesis
|October 1, 1994
PubMed
Summary

This study introduces a free computer program to statistically compare p53 gene mutation patterns in cancers. The tool helps determine if observed differences in mutation spectra between groups are significant.

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Area of Science:

  • Oncology
  • Genetics
  • Bioinformatics

Background:

  • Mutations in the p53 oncogene are frequent in human cancers.
  • Environmental factors may influence p53 mutation patterns, which vary across tumor types.
  • Assessing statistical significance of differing mutation spectra is challenging.

Purpose of the Study:

  • To develop and present a computational tool for comparing two p53 mutational spectra.
  • To provide a method for statistically evaluating differences in p53 mutation patterns.

Main Methods:

  • A computer program was developed for IBM-compatible personal computers.
  • The program analyzes text files containing mutation data (nature and number).
  • It outputs a P value to assess the probability of spectra originating from the same population.

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Main Results:

  • The program was demonstrated by comparing p53 mutation spectra in various cancer types and exposure groups.
  • Examples include bladder cancer (smokers vs. non-smokers), lung/colon cancers, and hepatocellular carcinoma (aflatoxin exposure).

Conclusions:

  • The developed program offers a statistically rigorous method for comparing p53 mutational spectra.
  • This tool aids in understanding the role of environmental mutagens and cancer type in p53 mutation patterns.
  • p53 mutation recognition differs from traditional mutational spectra experiments due to cancer's complex phenotype.