Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The monoclonal gammopathies

R A Kyle1

  • 1Division of Hematology and Internal Medicine, Mayo Clinic, Rochester, MN 55905.

Clinical Chemistry
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

Monoclonal proteins (M-proteins) are identified using electrophoresis and immunofixation. These methods aid in diagnosing conditions like multiple myeloma and macroglobulinemia.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medical mythology: Apollo.

Mayo Clinic proceedings·1992
Same author

Amyloidosis. Introduction and overview.

Journal of internal medicine·1992
Same author

Primary systemic amyloidosis.

Journal of internal medicine·1992
Same author

Wladyslaw Bieganski--father of modern Polish medicine.

Mayo Clinic proceedings·1992
Same author

Dialysis support of patients with primary systemic amyloidosis. A study of 211 patients.

Archives of internal medicine·1992
Same author

Medical mythology: Hera (Juno).

Mayo Clinic proceedings·1992

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Hematology

Background:

  • Monoclonal proteins (M-proteins) are composed of identical heavy and light polypeptide chains.
  • Various electrophoretic and immunochemical techniques are employed for their detection and characterization.

Purpose of the Study:

  • To outline the diagnostic utility of different methods for M-protein detection.
  • To differentiate M-proteins from other related hematologic disorders.

Main Methods:

  • Cellulose acetate membrane electrophoresis for screening M-proteins.
  • Agarose gel electrophoresis for enhanced sensitivity in detecting small M-proteins.
  • Immunoelectrophoresis and immunofixation for definitive diagnosis and equivocal cases.

Related Experiment Videos

Main Results:

  • Electrophoresis is effective for screening M-proteins.
  • Immunoelectrophoresis and immunofixation are crucial for diagnosing suspected disorders.
  • Bence Jones protein detection requires urine concentration and immunochemical analysis.

Conclusions:

  • Accurate M-protein identification is essential for diagnosing multiple myeloma, macroglobulinemia, and amyloidosis.
  • A combination of electrophoresis and immunochemical methods ensures comprehensive diagnostic evaluation.
  • Differential diagnosis includes benign monoclonal gammopathy and various lymphoproliferative disorders.