Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Snurposomes and coiled bodies

Z Wu1, C Murphy, C H Wu

  • 1Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210.

Cold Spring Harbor Symposia on Quantitative Biology
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and large volume production of extremely high current density YBa<sub>2</sub>Cu<sub>3</sub>O<sub>7</sub> superconducting wires for fusion.

Scientific reports·2021
Same author

A radiation hybrid comparative map of ovine chromosome 1 aligned to the virtual sheep genome.

Animal genetics·2009
Same author

Useful prediction of ureteral calculi visibility on abdominal radiographs based on calculi characteristics on unenhanced helical CT and CT scout radiographs.

International journal of clinical practice·2009
Same author

Pollutant removal within hybrid constructed wetland systems in tropical regions.

Water science and technology : a journal of the International Association on Water Pollution Research·2009
Same author

Recovery of heavy metals from industrial sludge using various acid extraction approaches.

Water science and technology : a journal of the International Association on Water Pollution Research·2009
Same author

The effects of strontium ranelate in Asian women with postmenopausal osteoporosis.

Calcified tissue international·2008

This study identifies novel prenucleolar bodies containing U3 small nucleolar RNA (snoRNA) and fibrillarin, crucial for pre-ribosomal RNA (rRNA) processing in amphibian oocytes. These structures function as pre-rRNA snurposomes, distinct from nucleoli, and are vital for early development.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Splicing small nuclear ribonucleoproteins (snRNPs) and U7 small nuclear RNA (snRNA) are found in amphibian oocyte germinal vesicles (GV) within snurposomes and C snurposomes.
  • U3 and U8 small nucleolar RNAs (snoRNAs), involved in pre-ribosomal RNA (rRNA) processing, are located in extrachromosomal nucleoli within the GV.
  • The existence of a snurposome containing U3 and U8 snoRNAs without rDNA or rRNA was investigated.

Purpose of the Study:

  • To identify and characterize novel snurposomes involved in pre-rRNA processing.
  • To investigate the composition and function of prenucleolar bodies in amphibian oocytes.

Main Methods:

  • Microscopic observation and characterization of extrachromosomal bodies in amphibian oocytes.

Related Experiment Videos

  • Analysis of snRNA and snoRNA localization within germinal vesicles.
  • Identification of prenucleolar bodies in telophase/early interphase nuclei and in vitro formed pronuclei.

    • Main Results:

    • A population of minute nucleoli, similar in size to B snurposomes, was identified.
    • Prenucleolar bodies in telophase/early interphase nuclei were found to contain U3 snoRNA and fibrillarin.
    • These prenucleolar bodies lack rDNA and do not synthesize rRNA, fitting the definition of pre-rRNA snurposomes.

    Conclusions:

    • Prenucleolar bodies represent a novel class of pre-rRNA snurposomes.
    • These structures are essential for early stages of ribosome biogenesis in amphibian oocytes.
    • Prenucleolar bodies share components with coiled bodies, suggesting conserved cellular machinery.