Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interpretation of tRNA-mischarging kinetics

A Dietrich, D Kern, J Bonnet

    European Journal of Biochemistry
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Atypical Resting-State EEG Graph Metrics of Network Efficiency Across Development in Autism and Their Association with Social Cognition: Results from the LEAP Study.

    Journal of autism and developmental disorders·2025
    Same author

    A biophysical framework for double-drugging kinases.

    bioRxiv : the preprint server for biology·2023
    Same author

    Bariatric surgery in liver cirrhosis.

    Frontiers in surgery·2023
    Same author

    Laparoscopic conversion of omega loop gastric bypass to Roux-en-Y gastric bypass for Barrett's esophagus: case report.

    BMC surgery·2022
    Same author

    High-Dosage Fosfomycin Results in Adequate Plasma and Target-Site Exposure in Morbidly Obese and Nonobese Nonhyperfiltration Patients.

    Antimicrobial agents and chemotherapy·2022
    Same author

    Risk of target non-attainment in obese compared to non-obese patients in calculated linezolid therapy.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020

    Incorrect transfer RNA (tRNA) aminoacylation reactions, like correct ones, are influenced by deacylation equilibria. This study shows mischarging kinetics are similar to correct aminoacylation, involving ATP hydrolysis.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Enzymology

    Background:

    • Incorrect tRNA aminoacylation is typically slow and incomplete.
    • Previous work suggested equilibria between aminoacylation and deacylation explain incomplete correct reactions.
    • The kinetics of mischarging, or incorrect aminoacylation, were not fully understood.

    Purpose of the Study:

    • To investigate the kinetic mechanisms underlying incorrect tRNA aminoacylation.
    • To determine if mischarging reactions are influenced by deacylation equilibria.
    • To compare the kinetics of correct and incorrect aminoacylation.

    Main Methods:

    • Kinetic analysis of valylation of yeast tRNAfMet by yeast valyl-tRNA synthetase.
    • Studying mischarging under standard and varied experimental conditions.

    Related Experiment Videos

  • Investigating other mischarging systems including yeast tRNAPhE and tRNA2Val.

    • Main Results:

    • Incorrect valylation of yeast tRNAfMet follows a kinetic rate law including aminoacylation and deacylation reactions.
    • Incomplete mischarging plateaus are attributed to equilibria involving valylation, deacylation, and reverse valylation.
    • The reverse reaction is less dominant at lower enzyme concentrations.
    • Similar kinetics were observed in other mischarging systems.
    • One ATP molecule is hydrolyzed per tRNA charged with valine during mischarging.

    Conclusions:

    • Correct and incorrect tRNA aminoacylation reactions exhibit similar kinetic behaviors.
    • Equilibria involving deacylation play a significant role in the observed kinetics of mischarging.
    • The findings provide a unified kinetic understanding of both correct and incorrect aminoacylation processes.