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Expression of the sarcoplasmic reticulum Ca(2+)-ATPase in yeast
F Centeno1, S Deschamps, A M Lompré
1Département de Biologie Cellulaire et Moléculaire, CEA, Gif sur Yvette, France.
FEBS Letters
|October 31, 1994
Abstract:
We describe here an easy system for the production of mg amounts of the rabbit Ca(2+)-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca(2+)-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.