Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

LDL subclasses in IDDM patients: relation to diabetic nephropathy

S Lahdenperä1, P H Groop, M Tilly-Kiesi

  • 1Third Department of Medicine, University of Helsinki, Finland.

Diabetologia
|July 1, 1994
PubMed
Summary

Diabetic nephropathy in patients with insulin-dependent diabetes mellitus (IDDM) is linked to increased LDL-cholesterol. This elevation is mainly due to a higher number of LDL particles, not changes in their size or composition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetically determined increase in apolipoprotein C-III (APOC3 gain-of-function) delays very low-density lipoprotein clearance in humans.

Atherosclerosis·2025
Same author

Acquired liver fat is a key determinant of serum lipid alterations in healthy monozygotic twins.

Obesity (Silver Spring, Md.)·2013
Same author

Response letter to D. Singh-franco et al.

Diabetes, obesity & metabolism·2012
Same author

Heritability and familiality of type 2 diabetes and related quantitative traits in the Botnia Study.

Diabetologia·2011
Same author

Determinants of urinary albumin excretion within the normal range in patients with type 2 diabetes: the Randomised Olmesartan and Diabetes Microalbuminuria Prevention (ROADMAP) study.

Diabetologia·2009
Same author

Genetic association analyses of non-synonymous single nucleotide polymorphisms in diabetic nephropathy.

Diabetologia·2008

Area of Science:

  • Endocrinology
  • Metabolic Syndrome
  • Nephrology

Background:

  • Diabetic nephropathy is a common complication of insulin-dependent diabetes mellitus (IDDM).
  • Elevated LDL-cholesterol is a known risk factor for cardiovascular disease in diabetic patients.
  • Understanding LDL particle characteristics in diabetic nephropathy is crucial for risk assessment.

Purpose of the Study:

  • To investigate changes in LDL particle size and composition in IDDM patients with varying stages of diabetic nephropathy.
  • To determine if elevated LDL-cholesterol in diabetic nephropathy is associated with altered LDL characteristics.

Main Methods:

  • Studied LDL particle distribution in normoalbuminuric, microalbuminuric, and proteinuric IDDM patients, and non-diabetic controls.
  • Utilized nondenaturing gradient gel electrophoresis to determine mean LDL particle diameter.

Related Experiment Videos

  • Employed density gradient ultracentrifugation to analyze LDL composition and density distribution.

    • Main Results:

    • Normoalbuminuric IDDM patients exhibited larger LDL particles compared to controls.
    • LDL particle diameter showed an inverse correlation with serum triglycerides across all groups.
    • Increased LDL mass in microalbuminuric and proteinuric IDDM patients was primarily attributed to an increase in lighter LDL particles.

    Conclusions:

    • The elevation of LDL mass in incipient and established diabetic nephropathy is mainly due to an increased number of LDL particles.
    • No significant changes in LDL density distribution or composition were observed between diabetic groups.
    • These findings suggest that increased LDL particle number, rather than altered LDL characteristics, contributes to elevated LDL-cholesterol in diabetic nephropathy.