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A recombinant DNA bio-assay for selenium in blood
1Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Gene
|October 21, 1994
Summary
This study introduces a novel bioassay to measure selenium (Se) status by quantifying functional selenocysteine (Sec) in selenoproteins. This method offers a sensitive and unique approach for assessing Se levels in biological fluids like blood serum.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Selenium (Se) is a crucial trace element incorporated as selenocysteine (Sec) into selenoproteins (SePro).
- Selenoproteins play vital roles in cell physiology and human health.
- Accurate assessment of Se status in biological fluids is essential.
Purpose of the Study:
- To develop a novel and sensitive bioassay for determining selenium status.
- To specifically measure functional selenocysteine residues within selenoproteins.
- To provide an adequate method for assessing Se status in biological samples.
Main Methods:
- A recombinant DNA construct was utilized in Escherichia coli.
- Expression of the 'lacZ gene was driven by UGA-directed Sec incorporation.
- Rat blood serum samples were analyzed after acid hydrolysis for protein degradation.
Main Results:
- A new bioassay (BIO) was established for Se status determination.
- The bioassay demonstrated proportional and specific correlation with Sec incorporation.
- This method uniquely quantifies all functional Sec residues in serum SePro.
Conclusions:
- The developed bioassay is a simple and sensitive tool for assessing Se status.
- This approach accurately measures functional Sec in selenoproteins from biological fluids.
- The bioassay provides a unique method for evaluating Se status in blood serum.