Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence of the sheep interleukin-10-encoding cDNA

B M Dutia1, P Hunt, D R Sargan

  • 1Department of Veterinary Pathology, University of Edinburgh, UK.

Gene
|November 18, 1994
PubMed
Summary

Researchers have sequenced the ovine interleukin-10 (oIL-10) gene, revealing its DNA and amino acid sequences. This finding provides insights into the structure and evolution of this important immune regulatory protein in sheep.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges of determining the relative contribution of determinants of health on population health: a Canadian perspective.

Perspectives in public health·2024
Same author

Real-world prevalence across 159 872 patients with cancer supports the clinical utility of TMB-H to define metastatic solid tumors for treatment with pembrolizumab.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Scrapie infection and endogenous retroviral expression in sheep lymphoid tissues.

Veterinary immunology and immunopathology·2021
Same author

Variation in zoobenthic blue carbon in the Arctic's Barents Sea shelf sediments.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2020
Same author

Burkholderia pseudomallei multi-centre study to establish EUCAST MIC and zone diameter distributions and epidemiological cut-off values.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

An open and closed case: timing of closure following laparostomy.

Annals of the Royal College of Surgeons of England·2020

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Interleukin-10 (IL-10) is a critical cytokine involved in immune regulation.
  • Understanding species-specific IL-10 sequences is vital for comparative immunology and disease research.

Purpose of the Study:

  • To clone and sequence the ovine interleukin-10 (oIL-10)-encoding cDNA.
  • To determine the complete coding sequence and predict the amino acid sequence of oIL-10.

Main Methods:

  • Gene amplification using the polymerase chain reaction (PCR).
  • DNA sequencing to determine the nucleotide sequence.
  • Bioinformatic analysis to predict amino acid sequence and protein characteristics.

Main Results:

Related Experiment Videos

  • The complete coding sequence of the oIL-10 gene is 531 nucleotides long.
  • The mature oIL-10 protein is predicted to consist of 158 amino acids (aa), with a molecular weight of 18,367 Da.
  • The oIL-10 protein shares significant sequence identity with IL-10 from other species, including pigs, humans, rodents, and viruses.

    • Conclusions:

    • The successful cloning and sequencing of oIL-10 provide a foundational resource for further immunological studies in sheep.
    • The high degree of sequence homology suggests conserved function across species.
    • This data aids in understanding the evolutionary relationships of IL-10 and its role in ovine immune responses.