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Characterization and distribution of insertion sequence IS1239 in Streptococcus pyogenes
Gene
|December 2, 1994
Summary
A novel insertion sequence, IS1239, was discovered in Streptococcus pyogenes. This mobile genetic element is present in 33% of isolates and may contribute to bacterial adaptation and virulence.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Streptococcus pyogenes is a significant human pathogen responsible for various infections.
- Streptococcal superantigen SSA is an extracellular product with homology to Staphylococcus aureus enterotoxins.
Purpose of the Study:
- To investigate allelic variation at the ssa locus in Streptococcus pyogenes.
- To identify and characterize novel genetic elements within S. pyogenes isolates.
Main Methods:
- Polymerase Chain Reaction (PCR) to detect insertions.
- Southern blot analysis to assess copy number.
- Sequence analysis to identify open reading frames and homology.
Main Results:
- A 1110-bp insertion sequence, designated IS1239, was identified in S. pyogenes isolates.
- IS1239 contains a 981-bp open reading frame with homology to IS30 transposase.
- IS1239 was detected in 33% of tested S. pyogenes isolates across different M serotypes.
Conclusions:
- IS1239 is the first simple insertion sequence identified in group-A streptococci.
- The widespread distribution of IS1239 suggests its mobility and potential role in S. pyogenes evolution.
- Further research is needed to understand the functional impact of IS1239 in S. pyogenes.