[Antibodies against Auerbach's plexus--studies using antisera with different fluorochromes]

Human sera containing autoantibodies against the plexus myentericus Auerbach were investigated to find the optimal conditions using double or triple immunofluorescence. FITC-, DTAF-, Texas-red-, tetramethylrhodamine-, Cy-3-, and AMCA-labeled antisera were used. The confirmation of the neuronal binding of the human antibody was achieved by the almost identical binding of monoclonal antibody against the 68 kD neurofilament protein. DTAF- and Cy-3-labeled antisera used as F(ab')2-fragments and absorbed repeatedly proved to be particularly suitable especially since they have a low fading effect.