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Related Experiment Videos

Cell-associated collagenolytic activity by group B streptococci

R J Jackson1, M L Dao, D V Lim

  • 1Department of Biology, University of South Florida, Tampa 33620-5150.

Infection and Immunity
|December 1, 1994
PubMed
Summary
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Group B streptococci (GBS) possess collagen-degrading enzymes that may contribute to premature rupture of membranes. This study identifies and characterizes GBS collagenolytic activity, potentially linking it to neonatal diseases.

Area of Science:

  • Microbiology
  • Biochemistry
  • Pathogenesis

Background:

  • Group B streptococci (GBS) are significant causes of neonatal infections, including sepsis, pneumonia, and meningitis.
  • GBS invasion of the placenta's amniotic membrane and potential role in premature rupture of membranes (PROM) have been observed.
  • Disordered collagen fibrils in the amnion in the presence of GBS suggest enzymatic activity.

Purpose of the Study:

  • To characterize the cell-associated collagenolytic activities of GBS.
  • To investigate the potential role of GBS collagenase in neonatal disease and PROM.

Main Methods:

  • Utilized sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Sephadex G-200 column chromatography, and gelatin zymograms.
  • Assessed the degradation of the synthetic collagen-mimicking peptide FALGPA by GBS.

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  • Investigated the effects of EDTA, 1,10-phenanthroline, heat, and anti-collagenase antiserum on GBS activity.

    • Main Results:

    • GBS USF704 hydrolyzed FALGPA, indicating collagenolytic activity.
    • Activity was inhibited by EDTA and 1,10-phenanthroline, characteristic of zinc metalloenzymes.
    • Heat inactivated the enzyme, while cell lysates showed higher activity.
    • Antiserum against Clostridium histolyticum collagenase cross-reacted with GBS proteins and inhibited FALGPA hydrolysis.
    • Twenty-five additional GBS isolates exhibited varying levels of FALGPA hydrolytic activity.

    Conclusions:

    • GBS possesses cell-associated collagenolytic activity.
    • This activity may contribute to the pathogenesis of premature rupture of membranes.
    • The findings suggest a potential mechanism for GBS-related neonatal disease.