Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cholesterol homeostasis. Modulation by amphiphiles

Y Lange1, T L Steck

  • 1Department of Pathology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.

The Journal of Biological Chemistry
|November 25, 1994
PubMed
Summary

Diverse amphiphiles modulate cellular cholesterol metabolism by altering cholesterol levels at regulatory sites. This research reveals two classes of compounds with opposing effects on cholesterol homeostasis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport.

Developmental biology·2000
Same author

Cholesterol movement in Niemann-Pick type C cells and in cells treated with amphiphiles.

The Journal of biological chemistry·2000
Same author

Regulation of endoplasmic reticulum cholesterol by plasma membrane cholesterol.

Journal of lipid research·1999
Same author

Four cholesterol-sensing proteins.

Current opinion in structural biology·1998
Same author

Circulation of cholesterol between lysosomes and the plasma membrane.

The Journal of biological chemistry·1998
Same author

Skipper, an LTR retrotransposon of Dictyostelium.

Nucleic acids research·1998

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Cellular cholesterol metabolism is crucial for maintaining homeostasis.
  • Amphiphilic molecules can influence cellular processes, including cholesterol regulation.
  • Oxysterols are known regulators of cholesterol metabolism.

Purpose of the Study:

  • To investigate the mechanisms by which diverse amphiphiles affect cellular cholesterol metabolism.
  • To elucidate the opposing effects of different amphiphile classes on cholesterol homeostasis.
  • To propose a general hypothesis for cellular cholesterol regulation.

Main Methods:

  • Treatment of cells with various amphiphiles, including oxysterols and a second class of agents (steroids, amines, etc.).
  • Measurement of cholesterol biosynthesis and plasma membrane cholesterol esterification.
  • Assessment of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity.

Main Results:

  • Oxysterols mimic excess cell cholesterol, inhibiting biosynthesis and stimulating esterification.
  • A second class of amphiphiles inhibits esterification and induces biosynthesis, affecting cholesterol regulatory proteins.
  • These agents modulate cholesterol levels at intracellular regulatory sites.

Conclusions:

  • Cellular cholesterol homeostasis is regulated by proteins in intracellular membranes sensitive to local cholesterol levels.
  • Cholesterol circulates from plasma membranes and lysosomes to intracellular sites, influencing regulatory protein activity.
  • Amphiphiles can disrupt or support cholesterol homeostasis by altering cholesterol availability at these regulatory sites.

Related Experiment Videos