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Related Experiment Videos

Regulated internalization of caveolae

R G Parton1, B Joggerst, K Simons

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

The Journal of Cell Biology
|December 1, 1994
PubMed
Summary
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Caveolae, specialized plasma membrane structures, dynamically internalize into cells. This process, influenced by kinase activity and actin networks, is reversible and crucial for cellular functions.

Area of Science:

  • Cell Biology
  • Membrane Biology
  • Biochemistry

Background:

  • Caveolae are plasma membrane invaginations involved in endocytosis and signal transduction.
  • Their dynamic behavior and internalization mechanisms are not fully understood.

Purpose of the Study:

  • To develop an assay to quantify internal versus plasma membrane caveolae.
  • To investigate the regulation of caveolae internalization.

Main Methods:

  • Developed an assay using GPI-anchored alkaline phosphatase clustering.
  • Utilized antibody-induced crosslinking, phosphatase inhibitors (okadaic acid), kinase inhibitors (staurosporine), and cytochalasin D.
  • Employed electron microscopy and hypertonic medium treatment.

Main Results:

Related Experiment Videos

  • Demonstrated antibody-induced, time- and temperature-dependent internalization of caveolae.
  • Okadaic acid accelerated internalization; cytochalasin D and staurosporine blocked it.
  • Electron microscopy confirmed okadaic acid-induced caveolae removal and redistribution, dependent on microtubules and actin.

Conclusions:

  • Caveolae are dynamic structures capable of internalization.
  • Caveolae internalization is regulated by kinase activity and requires intact actin and microtubule networks.