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Updated: Oct 9, 2026

Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
A competition immunoprecipitation assay of unlabeled poliovirus antigens
1Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Abstract:
An assay method is described for unlabeled, unpurified N(ative) and H(eated) antigens of poliovirus. The method is based on competition between the unlabeled antigen and a standard quantity of radiolabeled antigen, in the presence of a limiting amount of a N- or H-specific, monoclonal antibody. The immune complexes are removed by protein A-bearing, fixed staphylococci. The method is free from cross-reaction between N and H antigen, and has a detection limit of approximately 2 nM. It was applied successfully to the quantitation of poliovirus antigen synthesized by recombinant yeast expressing the viral proteins P1 and 3CD.
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