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Related Experiment Videos

C-erbB-2 immunostaining: problems with interpretation

E W Kay1, C J Walsh, M Cassidy

  • 1Department of Pathology, Royal College of Surgeons, Ireland.

Journal of Clinical Pathology
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

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Interobserver variability in c-erbB-2 immunostaining is significant, suggesting a need for standardized guidelines. Minimal intraobserver variability indicates individual pathologist consistency in assessing c-erbB-2 oncoprotein expression.

Area of Science:

  • Oncology
  • Pathology
  • Biomarker Research

Background:

  • The c-erbB-2 oncoprotein is a significant biomarker in various cancers.
  • Immunohistochemistry (IHC) is a common method for detecting c-erbB-2 expression.
  • Variability in IHC assessment can impact clinical decision-making.

Purpose of the Study:

  • To evaluate the consistency and reproducibility of c-erbB-2 IHC staining assessments.
  • To identify sources of inter- and intraobserver variability in c-erbB-2 scoring.
  • To characterize the spectrum of c-erbB-2 staining patterns across different tumor types.

Main Methods:

  • Examined 283 neoplasms for c-erbB-2 oncoprotein expression using IHC.
  • Three independent observers assessed membrane and cytoplasmic staining intensity (3- and 4-point scales) and extent (2-point scale).

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  • Assessments were repeated after a minimum two-week interval to evaluate intraobserver variability.

    • Main Results:

    • Membrane staining was observed in 16.6% of tumors, primarily in bladder, renal, and breast cancers.
    • Disagreement on membrane staining presence/absence was 11.3%.
    • Cytoplasmic staining was present in 55.5% of tumors, with 26.5% disagreement.

    Conclusions:

    • Intraobserver variability was low, suggesting consistent internal standards among pathologists.
    • Higher interobserver variability highlights the need for standardized c-erbB-2 IHC interpretation guidelines.
    • Recommendations include using standard controls, a 3-tier intensity scale, a 2-tier extent scale, and dual pathologist agreement for assessment. Cytoplasmic staining recording should continue pending further research.