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Related Experiment Videos

Astrocyte gene expression in experimental mouse scrapie

F Lazarini1, F Boussin, J P Deslys

  • 1Laboratoire de Neuropathologie Expérimentale et Neurovirologie, CRSSA, Commissariat à l'Energie Atomique, DPTE/DSV, Fontenay aux Roses, France.

Journal of Comparative Pathology
|July 1, 1994
PubMed
Summary

Scrapie prion protein accumulation in the brain correlates with increased glial fibrillary acidic protein (GFAP) expression, but not glutamine synthetase (GS) levels, in a mouse model of transmissible spongiform encephalopathies.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Prion Diseases

Background:

  • Transmissible spongiform encephalopathies (TSEs) are characterized by prion protein (PrPsc) accumulation in the brain.
  • Glial fibrillary acidic protein (GFAP) expression often increases with PrPsc accumulation.
  • Astrocyte gene expression changes during scrapie pathogenesis are not fully understood.

Purpose of the Study:

  • To investigate astrocyte gene expression, specifically glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), during scrapie development in a mouse model.
  • To compare the expression patterns of GFAP and GS mRNAs and proteins in relation to prion protein (PrP) and scrapie prion protein (PrPsc) accumulation.

Main Methods:

  • Intracerebral inoculation of newborn mice with scrapie.

Related Experiment Videos

  • Monitoring of scrapie progression over 172 days.
  • Quantitative analysis of PrP, GFAP, and GS mRNA and protein levels using Northern and Western blots.

    • Main Results:

    • GFAP mRNA showed a 10-fold increase from 112 to 172 days post-inoculation, with a 10-20 fold protein increase.
    • Glutamine synthetase (GS) mRNA showed a two-fold increase, but protein concentration remained constant.
    • Scrapie prion protein (PrPsc) was detected from day 84 and increased significantly by day 172.
    • Elevated GFAP levels were observed in scrapie-infected brains compared to controls.

    Conclusions:

    • GFAP and GS mRNAs are differentially regulated in the brain during scrapie infection.
    • GFAP upregulation is a prominent feature of astrocyte response in this scrapie mouse model.
    • GS levels do not consistently reflect scrapie progression, unlike GFAP.