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Related Experiment Videos

Nerve growth factor production by lymphocytes

L Santambrogio1, M Benedetti, M V Chao

  • 1Department of Pathology, New York University School of Medicine, NY 10016.

Journal of Immunology (Baltimore, Md. : 1950)
|November 15, 1994
PubMed
Summary
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Lymphocytes, including T and B cells, synthesize and secrete active nerve growth factor (NGF). This secreted NGF can impact both the immune system and peripheral neurons.

Area of Science:

  • Immunology
  • Neuroscience
  • Molecular Biology

Background:

  • Nerve growth factor (NGF) is crucial for neuronal development and survival.
  • Emerging evidence suggests NGF also influences immune cells.
  • The non-neuronal sources of NGF require further investigation.

Purpose of the Study:

  • To identify extra-neural sources of NGF.
  • To investigate NGF synthesis and secretion by immune cells.
  • To determine the functional activity of lymphocyte-derived NGF.

Main Methods:

  • Detection of NGF mRNA in lymphocytes using RNase protection assay, PCR, and DNA sequencing.
  • Analysis of NGF protein expression and secretion via Western blot.
  • Assessment of secreted NGF activity on PC12 cells and 3T3 fibroblasts.

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Main Results:

  • NGF-specific mRNA was detected in mouse CD4+ and CD8+ T lymphocytes, splenic B lymphocytes, and a lymphoma cell line.
  • Antigenic stimulation increased NGF mRNA in Th2 clones.
  • Secreted NGF was biologically active, inducing PC12 cell differentiation and tyrosine kinase autophosphorylation.

Conclusions:

  • Lymphocytes synthesize and secrete functional NGF.
  • Lymphocyte-derived NGF may act as a para-autocrine factor within the immune system.
  • This NGF could also contribute to the maintenance of peripheral neurons.