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Related Experiment Videos

A protective "concealed" antigen from Boophilus microplus. Purification, localization, and possible function

G A Riding1, J Jarmey, R V McKenna

  • 1CSIRO Division of Tropical Animal Production, Indooropilly, Queensland, Australia.

Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1994
PubMed
Summary
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Researchers identified a novel membrane protein, Bm91, effective for vaccinating cattle against the cattle tick Boophilus microplus. This protein, found in tick salivary glands and gut, offers a new strategy against tick infestations.

Area of Science:

  • Veterinary Parasitology
  • Molecular Biology
  • Immunology

Background:

  • Cattle ticks like Boophilus microplus cause significant economic losses in livestock.
  • Vaccination presents a sustainable alternative to chemical acaricides for tick control.
  • Identifying effective antigens from ticks is crucial for developing successful vaccines.

Purpose of the Study:

  • To purify and characterize a membrane protein from Boophilus microplus for potential use in cattle vaccination.
  • To investigate the properties and potential function of the identified protein.
  • To assess the novelty of the antigen for inducing immunity against ticks.

Main Methods:

  • Purification of the Bm91 membrane protein from semi-engorged adult female ticks.
  • Characterization of Bm91's molecular weight, isoelectric point, and glycosylation.

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  • Partial amino acid sequencing to determine homology with known proteins.
  • Analysis of Bm91 localization within tick tissues (salivary gland and gut).

    • Main Results:

    • The mature Bm91 protein has an apparent molecular weight of 86,000 and an isoelectric point of 4.8-5.2.
    • Bm91 is glycosylated and binds to lentil lectin, indicating its presence in the salivary gland and gut.
    • Amino acid sequence data suggests Bm91 is similar to mammalian angiotensin-converting enzyme, implying a potential enzymatic function.
    • Cattle sera from naturally exposed animals did not recognize Bm91, suggesting it is a 'concealed' antigen.

    Conclusions:

    • Bm91 is a promising candidate antigen for developing a vaccine against Boophilus microplus.
    • The 'concealed' nature of Bm91 may explain its effectiveness in inducing immunity.
    • This research contributes to the development of novel strategies for tick control in cattle.