Identification of Gomori-positive glia in autoradiographs

Unstained Gomori or paraldehyde fuchsin or chrome-haematoxylin-alum positive granules in the perikaria of so-called Gomori-positive glia of the periventricular zone of the rat hypothalamus, fixed with Bouin's or Carnoy's fluids, have been found to produce a positive chemographic (blackening) effect on autoradiographic emulsion. Similar action was produced by some granules in ependymal cells localized in the ventral part of the third ventricle between optical chiasma and median eminence. It is suggested that this positive chemographic effect can be employed for the identification and characterization of Gomori-positive glia in autoradiographic studies.