Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Invasive aspergillosis: clusters and sources?

K W Loudon1, A P Coke, J P Burnie

  • 1Department of Medical Microbiology, University of Manchester Medical School, UK.

Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation.

Genome biology·2020
Same author

The renal channelopathies.

Annals of clinical biochemistry·2014
Same author

The rho-kinase inhibitors Y-27632 and fasudil act synergistically with imatinib to inhibit the expansion of ex vivo CD34(+) CML progenitor cells.

Leukemia·2007
Same author

HLA-DR15, reduced relapse rate and improved survival after HLA identical sibling hemopoietic stem cell transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation·2007
Same author

Comparison of gene-expression profiles in parallel bone marrow and peripheral blood samples in acute myeloid leukaemia by real-time polymerase chain reaction.

Journal of clinical pathology·2006
Same author

Human recombinant antibody to HSP90: a natural partner in combination therapy.

Current molecular medicine·2005

Clusters of invasive Aspergillus fumigatus infections linked to building work were studied using three typing methods. Three patients shared an indistinguishable fungal strain, indicating a common source that should be identified and removed to control outbreaks.

Area of Science:

  • Medical Mycology
  • Infectious Disease Epidemiology
  • Molecular Typing

Background:

  • Invasive infections caused by Aspergillus fumigatus are a significant concern, particularly in immunocompromised patients.
  • Outbreaks of Aspergillus fumigatus have been associated with construction or renovation activities within healthcare facilities.
  • Studying the epidemiology of these infections has been challenging due to the absence of a robust typing system.

Purpose of the Study:

  • To investigate the epidemiological links between building works and invasive Aspergillus fumigatus infections.
  • To evaluate the utility of a combination of molecular typing techniques for identifying the source of infection.
  • To assess the feasibility of controlling Aspergillus fumigatus outbreaks through source detection and removal.

Main Methods:

Related Experiment Videos

  • A combination of three typing systems was employed: silver staining of sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE), immunoblot fingerprinting, and random amplification of polymorphic DNA (RAPD).
  • These methods were applied to Aspergillus fumigatus isolates from seven cases admitted to a haematology unit.
  • The typing results were analyzed to determine the relatedness of the isolates.

Main Results:

  • The study identified three patients with indistinguishable Aspergillus fumigatus isolates.
  • This finding strongly suggests a common source of infection among these three individuals.
  • The applied typing methods proved effective in differentiating and linking fungal isolates.

Conclusions:

  • A common source of Aspergillus fumigatus infection was identified in a cluster of cases on a haematology unit.
  • Effective control of invasive Aspergillus fumigatus infections associated with building works requires diligent source detection and removal.
  • Despite the challenges, identifying and eliminating sources is a crucial strategy for preventing and managing these infections.