Related Experiment Video
Updated: Aug 20, 2026

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
Published on: October 13, 2011
Topological differences in the expression of collagen type I and collagen type III mRNAs in the rat gingiva
M Redlich1, I Peleg, H Cooperman
1Connective Tissue Research Laboratory, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
Abstract:
Collagen mRNA levels in the gingival cells of molars and incisors in rats were measured and correlated with the ratio of interstitial collagen to DNA in these regions. Hybridization of 32P-labeled specific cDNA probes for collagen types I and III with total RNA isolated from gingival tissue of rat molars and incisors showed that the steady-state levels of mRNAs of type I was significantly higher in the molars than in the incisors (molars/incisors = 2.12 +/- 0.12, P < 0.004). However, the ratio of interstitial collagen to DNA in the gingiva of the molars was significantly lower than that found in the incisors (collagen/DNA = 4.13 +/- 0.90 and 12.89 +/- 1.24 respectively, P < 0.001). It is suggested that the difference between the mRNA levels and those of interstitial collagen may reflect an intrinsic characteristic presumably associated with the different modes of mastication between molars and incisors of the rat.
Related Concept Videos
Collagens are the Major Structural Proteins of ECM
Connective tissue proper includes loose...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Type IV Collagen of Basal Lamina
A type IV collagen molecule has six alpha chains which can exist in...
Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...

