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Antibiogram-resistogram typing scheme for methicillin-resistant Staphylococcus aureus

A S Rossney1, D C Coleman, C T Keane

  • 1Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, Trinity College Dublin, St James's Hospital, Ireland.

Journal of Medical Microbiology
|December 1, 1994
PubMed
Summary
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A new antibiogram-resistogram (AR) typing scheme rapidly differentiates methicillin-resistant Staphylococcus aureus (MRSA) isolates. This method is more discriminatory than traditional techniques and provides results within 24 hours for laboratory use.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Accurate and rapid differentiation of methicillin-resistant Staphylococcus aureus (MRSA) is crucial for infection control.
  • Existing typing methods like phage typing and plasmid profiling have limitations in speed and discriminatory power.

Purpose of the Study:

  • To develop and evaluate a simple, rapid antibiogram-resistogram (AR) typing scheme for MRSA isolates.
  • To compare the discriminatory capacity of the AR scheme with established typing methods.

Main Methods:

  • Disk diffusion testing was used to determine susceptibility to 18 antibiotics and 4 chemicals.
  • A modified Stokes' technique with a specific control strain was employed.
  • AR typing was compared against phage typing and plasmid profiling.

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Main Results:

  • The AR typing scheme effectively differentiated MRSA isolates.
  • It proved to be more discriminatory than both phage typing and plasmid profiling.
  • Confirmatory tests identified a shared AR pattern between two strains, distinguishable by minocycline susceptibility.

Conclusions:

  • The developed AR typing scheme offers a simple, rapid, and highly discriminatory method for MRSA identification.
  • It is suitable for routine use in diagnostic laboratories, yielding results within 24 hours.