Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spectrin in developing normal and cardiomyopathic hamster heart

D A Messina1, L F Lemanski

  • 1Department of Anatomy and Cell Biology, Suny, Syracuse 13210.

Journal of Molecular and Cellular Cardiology
|July 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel protein involved in heart development in Ambystoma mexicanum is localized in endoplasmic reticulum.

Journal of biomedical science·2008
Same author

Molecular and immunohistochemical analyses of cardiac troponin T during cardiac development in the Mexican axolotl, Ambystoma mexicanum.

Journal of cellular biochemistry·2006
Same author

Changes in myofibrils and cytoskeleton of neonatal hamster myocardial cells in culture: an immunofluorescence study.

Tissue & cell·2005
Same author

Downregulation of N1 gene expression inhibits the initial heartbeating and heart development in axolotls.

Tissue & cell·2004
Same author

Relationship between cardiac protein tyrosine phosphorylation and myofibrillogenesis during axolotl heart development.

Tissue & cell·2003
Same author

Expression of HoxA5 in the heart is upregulated during thyroxin-induced metamorphosis of the Mexican axolotl (Ambystoma mexicanum).

Cardiovascular toxicology·2002

Spectrin distribution in normal and cardiomyopathic hearts showed no significant differences. This suggests spectrin is not a factor in the myofibril and membrane defects observed in these cardiac conditions.

Area of Science:

  • Cardiovascular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Spectrin, a cytoskeletal-membrane protein, plays a role in various cell types, but its function in non-erythroid tissues remains unclear.
  • Previous research indicated spectrin's association with membranes, myofibrils, intercalated discs, and T-tubules in developing normal Syrian hamster hearts.
  • Cardiomyopathic (CM) Syrian hamster hearts present with known myofibril and membrane abnormalities, making them a relevant model for studying spectrin's role.

Purpose of the Study:

  • To investigate the presence and distribution of spectrin in normal and cardiomyopathic (CM) hearts across different developmental stages.
  • To determine if spectrin is altered in CM hearts and could contribute to observed cardiac defects.

Main Methods:

  • Immunohistochemical analysis to visualize spectrin distribution.

Related Experiment Videos

  • Electron microscopy for ultrastructural examination of cardiac tissues.
  • Electrophoretic techniques to assess spectrin presence and quantity.

    • Main Results:

    • No discernible differences in spectrin distribution were observed between normal and CM hearts.
    • The specific spectrin isoform detected by the antibody was unaffected by the cardiomyopathic condition.

    Conclusions:

    • The spectrin isoform studied does not appear to be involved in the myofibril disarray or membrane defects characteristic of this model of cardiomyopathy.
    • Spectrin can be excluded as a direct contributor to the pathogenesis of cardiac abnormalities in this CM hamster model.