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Optimal lengths for DNAs encapsidated by Epstein-Barr virus

T A Bloss1, B Sugden

  • 1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

Journal of Virology
|December 1, 1994
PubMed
Summary

Epstein-Barr virus (EBV) DNA packaging efficiency is influenced by DNA length. EBV selects specific DNA lengths for packaging into virions, differing from cellular DNA, and impacts viral DNA size.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Epstein-Barr virus (EBV) is a human herpesvirus known to establish lifelong infections.
  • Understanding the mechanisms of EBV DNA packaging is crucial for comprehending viral replication and pathogenesis.

Purpose of the Study:

  • To investigate how DNA length affects the efficiency of DNA packaging by Epstein-Barr virus (EBV).
  • To determine if EBV selects specific DNA lengths for packaging into mature virions.

Main Methods:

  • Constructing plasmids with EBV replication origins (oriP, oriLyt) and terminal repeats, incorporating varying lengths of bacteriophage lambda DNA.
  • Introducing these plasmids into EBV-positive cells and inducing the lytic phase.
  • Analyzing the lengths of replicated plasmid concatemers within cells and packaged within mature virions.

Main Results:

  • Certain lengths of plasmid-derived concatemers were found to predominate in packaged virions.
  • The distribution of DNA lengths in virions differed significantly from that within the host cell.
  • The packaged B95-8 viral DNA was measured to be approximately 165 kbp, shorter than predicted.

Conclusions:

  • EBV DNA packaging efficiency is dependent on the length of the DNA.
  • EBV selectively packages a subset of available DNA lengths, indicating a length-dependent selection process.
  • Both viral terminal repeats and DNA length are critical, imprecisely recognized elements in EBV DNA packaging.

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