Related Experiment Video
Updated: Aug 10, 2026
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
Measurement of intracellular pH in isolated human detrusor smooth muscle cells
C H Fry1, C R Gallegos, B S Montgomery
1Department of Physiology, United Medical School, London, United Kingdom.
Abstract:
Intracellular pH, pHi, was measured in isolated human detrusor smooth muscle cells by epifluorescence microscopy. The mean value was 7.07 +/- 0.19 when superfused with a CO2/HCO3- solution. Alteration of superfusate PCO2 also changed pHi whereas NaHCO3 changes were without effect, suggesting that the cell membrane is permeable to CO2 but impermeable to HCO3-. Nonbicarbonate H+ buffering power of the cell was calculated as 20.3 mequiv. x 1(-1) x pH unit-1. Addition of NH4Cl to the superfusate induced an alkalosis and, upon removal, a transient acidosis. The recovery from the acidosis allowed calculation of the acid extrusion rate from the cell. The rate was dependent on pHi and increased as the cell was more acid.
More Related Videos
11:54Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
Published on: August 11, 2017
08:30Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
Published on: January 31, 2020